Confirmed Characters (villagers & special characters):
Bill | Curt | Punchy | Bunnie | Pecan | Joey | Bob | Filbert | Rosie | Static | Lily | Nibbles | Gaston | Pompom | Buck | Mac
Tom Nook | Shizu (Secretary)
Confirmed Bugs / Fish / Fossils / Paintings:
Tiger Butterfly | Yellow Butterfly
[No fish, fossils, or paintings confirmed yet]
Confirmed Flowers / Bushes / Fruit / Trees / Tools / Other:
Red Pansies | White Pansies | Yellow Pansies | Red Tulips | White Tulips | Yellow Tulips | Red Roses | White Roses | Yellow Roses
Green Bushes | Blue Flower Bushes | Purple Flower Bushes
Apple | Pear | Cherry | Orange | Peach | Coconut
Regular Tree | Autumn Tree | Cedar Tree | Fruit Tree | Coconut Tree | Stump
Net | Fishing Rod | Watering Can
Rocks | Mushrooms | Weeds | Clovers | Shells | Benches | Lamp Posts | Clock Posts | Waterfalls | *Wooden Structure
* - This wooden structure is shown in the Animal Crossing 3DS E3 2011 trailer and is surrounded by flowers. Its rumored purpose is to attract certain bugs.